Urea Page Gel - For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels. Pour gel to ~ 0.5 cm from top. Web tbe gels are used for dsdna analysis, to assess the purity of pcr products and for rnase protection assays. Push all the way down, but don't trap any bubbles. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. Add 25 μl temed and 50 μl 25% aps. Insert clean, dry comb at an angle to prevent trapping of bubbles.
Nucleic acids from 50 to 2,000 bp to are efficiently separated on tbe gels. Pour gel to ~ 0.5 cm from top. Add 25 μl temed and 50 μl 25% aps. Insert clean, dry comb at an angle to prevent trapping of bubbles. Push all the way down, but don't trap any bubbles. Vigorously agitate the solution by magnetic stirring to ensure complete mixing and solving of urea powder. For a denaturing 10% polyacrylamide gel solution of 40 ml, mix the following: Web tbe gels are used for dsdna analysis, to assess the purity of pcr products and for rnase protection assays.